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5 . 2012

Subpopulation content of lymphocytes in peripheral blood in patients with diabetes mellitus type 2 and obesity

AbstractIn the present study was determined the relative quantity subpopulations of lymphocytes, activated T-lymphocytes and CD95-antigen (Fas/APO-1) expression on lymphocytes in the peripheral blood of patients with diabetes mellitus type 2 (DM 2) and obesity in comparison with the healthy persons. The cells were analyzed by the method of flow cytometry using Beckman Coulter FC 500 cytometer. The following of cells subsets: CD19+, CD3+, CD3+CD4+, CD3+CD8+, CD3–CD16+СD56+, CD3+CD16+CD56+, CD3+CD25+, CD3+HLA-DR+, CD45+CD95+ were investigated. In this research was establish the rise of immunoregulatory index (CD3+CD4+/CD3+CD8+) in consequence of decrease the percentages of cytotoxic T-lymphocytes in patients with DM2 and obesity in comparison with the healthy persons. In the peripheral blood of patients with DM2 and obesity were observed a greater level of activated T-lymphocytes (CD3+HLA-DR+), that reflect the increase activity of T-cell immunity. The direct correlation was found between the increased quantity of NKT-cells (CD3+CD16+CD56+) and contain of triglicerides in the peripheral blood of patients with DM2. In these patients also was noted a greater level of lymphocytes expression of CD95-antigen in comparison with the patients with obesity and healthy persons. These results suggest that immune mechanisms play the important role in pathogenesesis of DM2 and obesity.

Keywords:diabetes mellitus type 2, obesity, subpopulations of lymphocytes, markers of activation, cell immunity

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CHIEF EDITOR
CHIEF EDITOR
Viktor A. Tutelyan
Full Member of the Russian Academy of Sciences, Doctor of Medical Sciences, Professor, Scientific Director of the Federal Research Centre of Nutrition, Biotechnology and Food Safety (Moscow, Russia)

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