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2 . 2013

Effects of omega-3 polyunsaturated fatty acids on antioxidant capacity in rats

AbstractDietary administration of omega-3 polyunsaturated fatty acids (EPA + DHA, 1,2:1) at dose 0,3 or 1 g/kg bw during 4 weeks led to minor (by 14% and 17%, p<0,05) decrease of serum antioxidant capacity and serum level of vitamin E (by 30% and 31%, p<0,05) and the activity of paraoxonase-1 (by 14% at 0,3 g/kg bw, p<0,05). The activity of antioxidant enzymes in liver increasedin a dose-dependent manner. At higher dose of omega-3 polyunsaturated fatty acids a 45% increase in the activity of paraoxonase-1 (p<0,05), 21% – heme oxygenase-1 (p>0,05), 68% – quinone reductase (p<0,05), 19% – glutathione S-transferase (p<0,05) compared to the control group was found. The direct relationship between activities of enzymes and increase of MDA level in liver (by 47 and 107%, p<0,05) was found. Selected doses of omega-3 polyunsaturated fatty acids had no significant effects on total liver antioxidant capacity, microsomes NADPH-Fe2+-induced lipid peroxidation and lysosomal membranes stability.

Keywords:omega-3 PUFA, eicosapentaenoic acid, docosahexaenoic acid, paraoxonase-1, heme oxygenase-1, quinone reductase, glutathione S-transferase, vitamins A and E

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