Change of blood antioxidant capacity of experimental animals during nutritional correction under oxidative stress
AbstractThe effect of nutritional correction (a diet high in foods with antioxidant content) on blood parameters in laboratory animals with metabolic disorders associated with oxidative stress has been studied. In experimental models of laboratory animals (male rabbits weighing 3,5–4,0 kg, n=40) with purulent septic diseases it has been demonstrated that the use of nutritive correction (replacement of 100 g of the cereal mixture through day on a mixture of cabbage 50 g, carrots 50 g, beet 25 g, apple 25 g, kiwi 10 g and garnet 10 g per 1 rabbit) is not inferior to its efficiency of glutathione use (2 g per day). The use of these antioxidants in laboratory animals significantly reduced the phenomenon of oxidative stress on the 5th day: blood antioxidant capacity significantly increased by 14,9 and 26,6%, and the area of the flash of luminol-dependent H2O2-induced chemiluminescence of blood plasma reduced by 44,2 and 48,6% in the experimental groups receiving respectively nutritive correction and glutathione. The low-molecula level of blood antioxidant capacity was restored and the balance of the activity of superoxide dismutase (decrease) and catalase (increase) was achieved on the 10th day of the experiment. These figures significantly (p<0,05) differed from than in the group of animals receiving no antioxidant correction. The latter studied parameters of prooxidant-antioxidant system reached values comparable with those in intact animals (n=10) only on the 30th day, confirming the advisability of appointing a complex antioxidant therapy.
Keywords:antioxidant system, energy potential, oxidative stress, nutrients