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5 . 2014

Complex of zinc with enzymatic hydrolysate of pigspleen protein – in vivo investigation

Abstract

Experimental sample of complex zinc with enzymatic hydrolysate of pigspleen protein (Zn-EHPS) was produced in laboratory with ultra- and nanofiltration technologies; its composition was analyzed; male rats Wister (initial body weight 132Ѓ}2,4 g) were under observation in vivo: rats were given zinc-deficient ration (ZDR) ≪Zinc Deficient Diet, Egg White Base≫, containing not more than 1,3 mg zinc/kg, and water ad libitum during 18 days. Animals were divided into 3 groups (10 in each): group 1 was given ZDR and distilled water; group 2 – ZDR and water zinc sulphate solution (ZnSO4) with zinc concentration of 16,3 mcg/ml; group 3 – ZDR and water Zn-EHPS solution with zinc concentration of 16,3 mcg/ml. All rats were daily observed with registration of water consumption. Unconditioned reflex (photophobia) or crossing time from light to darkness on special system before the beginning of experiment was tested out.

An increase of body weight (ΔW) during the experiment, unconditioned reflex stability and training to conditioned passive avoidance reflex (CPAR) after paw painful electric stimulation were studied. On 18 day in 16 hours after paw painful electric stimulation alkaline phosphatase activity (AP) and corticosterone concentration in rat blood serum were examined. Insignificant changes in distribution of peptide fractions according its molecular mass and practical complete remove of free metal ions from final product were observed when Zn-EHPS-technology were used. During experiment in all groups correspondently ΔW was 23, 87 and 82% (p<0,05); AP activity – 1,70Ѓ}0,06; 3,10Ѓ}0,25; 4,19Ѓ} 0,26 mcmol/l/s (p<0,01); corticosterone concentration – 20,7Ѓ}7,2; 21,5Ѓ}6,3; 22,1Ѓ}6,5 ng/ml (p>0,1) were observed. Significant (p<0,05) worse change of unconditioned reflex (photophobia) in group 1 (ZDR) in comparison with group 2 and group 3 (latent period – LP, s: 50,9Ѓ}12,0; 18,2Ѓ}16,0; 16,0Ѓ}4,2) as well as to initial test (LP, s – 16,8Ѓ}8,0) was found on the 14th day of the experiment.

According to LP-test CPAR in all groups in 24 and in 96 hours after paw painful electric stimulation was found to be maintained. Data obtained let to suggest high bioavailability of new zinc organic form – Zn-EHPS and its usage possibility in fortification of special foods.

Keywords:enzymatic hydrolysis, ultra-and nanofiltration technology, essential microelement, zinc, pig spleen, passive avoidance

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CHIEF EDITOR
CHIEF EDITOR
Viktor A. Tutelyan
Full Member of the Russian Academy of Sciences, Doctor of Medical Sciences, Professor, Scientific Director of the Federal Research Centre of Nutrition, Biotechnology and Food Safety (Moscow, Russia)

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