To the content
1 . 2017

Efficiency of polyphenol-rich grape products for the prevention of metabolic disorders in the experiment

Abstract

The article presents the results of study of the effect of polyphenol-rich materials obtained from grapes on the clinical symptoms, activity of non-specific proteases, and parameters of lipid peroxidation (LPO) of blood of rats with fructose model of metabolic syndrome (MS). White male rats (n=54, with initial body weight of 190-210 g) were randomly divided into five groups: control and 4 experimental groups. Body weight, circumference of the abdomen, blood serum level of glucose, total cholesterol, triglycerides and high density (HDL) lipoproteins were monitored in all animals in dynamics. Also non-specific proteases and their inhibitors were evaluated in rat blood by enzymatic methods, lipid peroxidation profile (malondialdehyde and caeruloplasmin blood serum level, superoxide dismutase blood activity) - by spectrophotometric methods. Modeling of the MS (10% fructose in drinking water) within 8 weeks resulted in statistically significant increase in body mass, abdominal adipose tissue, the activity of elastase-like (ELA) and trypsin-like (TLA) proteinases (20 and 18% respectively), the level of secondary products of LPO (50%), and decrease in activity of superoxide dismutase (15%) compared with the parameters of intact animals. The use of polyphenol-rich materials obtained from grapes with a total content of 1; 1,1 and 4 mg of phenolic compounds (in 0.05 ml) at MS for 4 weeks contributed regression the key clinical signs of MS: significant decrease of glucose and triglyceride levels against the background of increasing the HDL cholesterol were observed. The polyphenol-rich materials obtained from grapes influenced positively on the proteolytic profile (decreased TLA and ELA by 20% at the maximum dosage), contributed to the increase of the level of acid-stable protease inhibitors (by 21%), and reduced the amount of secondary products of LPO (by 34%). The dose-dependent effect of the amount of polyphenol components contained in food concentrates has been revealed.

Keywords:metabolic syndrome, peroxidation, polyphenols from grapes, protease, inhibitors

Voprosy pitaniia [Problems of Nutrition]. 2017; 86 (1): С. 100-107. doi: 10.24411/0042-8833-2017-00027.

All articles in our journal are distributed under the Creative Commons Attribution 4.0 International License (CC BY 4.0 license)

SCImago Journal & Country Rank
Scopus CiteScore
CHIEF EDITOR
CHIEF EDITOR
Viktor A. Tutelyan
Full Member of the Russian Academy of Sciences, Doctor of Medical Sciences, Professor, Scientific Director of the Federal Research Centre of Nutrition, Biotechnology and Food Safety (Moscow, Russia)

Journals of «GEOTAR-Media»