To the content
6 . 2019

The study of the polyphenolic complex and iridoid glycosides in various cultivars of edible honeysuckle fruits Lonicera edulis Turcz. ex Freyn

Abstract

The high content of minor biologically active substances in the fruits of wild-growing and cultivated varieties of honeysuckle, along with the low knowledge of the chemical composition of domestic varieties, determines the relevance of the study of their qualitative and quantitative composition.

The aim of the work was to study the detailed composition of the main groups of biologically active polyphenolic compounds [anthocyanins, flavonoids, hydroxycinnamic acids (HCAs), proanthocyanidins] and iridoids in various cultivars of domestic edible honeysuckle fruits (Lonicera edulis Turcz. Ex Freyn).

Material and methods. 15 samples of frozen fruits of edible honeysuckle harvested in Tambov, Voronezh, Moscow regions and Karelia were investigated. The total content of polyphenolic compounds (in terms of gallic acid equivalents) was determined by the modified Folin-Ciocalteu method, the total content of monomeric anthocyanins (in terms of cyanidin-3-glucoside) - by pH-differential spectrophotometry, proanthocyanidins (in terms of procyanidin B2) - by the modified Bate-Smith method. The profiles of individual anthocyanins, iridoids, flavonoids and HCAs was determined by HPLC-DAD/TOF-MS. The carbohydrate profile by capillary electrophoresis and antiradical activity in DPPH test in vitro were investigated as well.

Results and discussion. The main groups of polyphenolic compounds were anthocyanins and proanthocyanidins. Cyanidin-3-glucoside was found as predominant among anthocyanins (>85% of their sum). Substantial amounts of iridoids (from 78.0 till 341.8 mg/100 g) were found in the honeysuckle examined. The loganic acid and loganine prevailed among iridoids. The total content of flavonoids varied in the range of 9.246.6 mg/100 g, the main of which was rutin. Among HCAs chlorogenic acid prevailed, which accounted for 85.7-90.4% of the total amount of HCAs (45.9-79.8 mg/100 g). A correlation was found between the amount of polyphenolic compounds and the antiradical properties of honeysuckle fruits in DPPH test.

Conclusion. Based on the results of the study the most promising varieties of domestic honeysuckle in terms of the highest content of polyphenolic antioxidants and iridoids with potential anti-inflammatory, hypoglycemic, hypolipidemic, antimicrobial and other types of biological activity were determined for the first time.

Keywords:blue honeysuckle (Lonicera edulis Turcz. ex Freyn), anthocyanins, iridoids, proanthocyanidins, flavonoids, chlorogenic acid, DPPH

For citation: Perova I.B., Rylina E.V., Eller K.I., Akimov M.Yu. The study of the polyphenolic complex and iridoid glycosides in various cultivars of edible honeysuckle fruits Lonicera edulis Turcz. ex Freyn. Voprosy pitaniia [Problems of Nutrition]. 2019; 88 (6): 88-99. doi: 10.24411/0042-8833-2019-10069 (in Russian)

References

1. Bonarska-Kujawa D., Pruchnik H., Cyboran S. Biophysical mech- 4. anism of the protective effect of blue honeysuckle (Lonicera caeru-lea L. var. kamtschatica Sevast.) polyphenols extracts against lipid peroxidation of erythrocyte and lipid membranes. J Membrane Biol. 2014; 247: 611-25.

2. Jurikova T., Rop O., Mlcek J., et al. Phenolic profile of edible honeysuckle berries (genus Lonicera) and their biological effects. Molecules. 2012; 17: 61-79.

3. Fomenko S.E., Kushnerova N.F., Sprygin V.G., et al. Hepatorotective activity of honeysuckle fruit extract in carbon tetrachloride intoxicated rats. Eksperimental’naia i Klinicheskaia Farmakolo-giia [Experimental and Clinical Pharmacology]. 2014; 77 (10): 26-30. (in Russian)

4. Kula M., Majdan M., Radwanska A., et al. Chemical composition and biological activity of the fruits from Lonicera caerulea var. edulis "Wojtek". AJMP (Academia Journal of Medicinal Plants). 2013; 1 (8): 141-8.

5. Dinda B., Chowdhury D. R., Mohanta B.C. Naturally occurring iridoids, secoiridoids and their bioactivity. An updated review, part 3. Chem Pharm Bull. 2009; 57 (8): 765-96.

6. Ren M.-T., Chen J., Song Y., et al. Identification and quantification of 32 bioactive compounds in Lonicera species by high performance liquid chromatography coupled with time-of-flight mass spectrometry. J Pharm Biomed Anal. 2008; 48: 1351-60.

7. Whitehead S.R., Bowers M.D. Iridoid and secoiridoid glycosides in a hybrid complex of bush honeysuckles (Lonicera spp., Caprifolicaceae): Implications for evolutionary ecology and invasion biology. Phytochemistry. 2013; 86: 57-63.

8. Wu X., Gu L., Prior R.L., et al. Characterization of anthocyanins and proanthocyanidins in some cultivars of Ribes, Aronia, and Sambucus and their antioxidant capacity. J Agric Food Chem. 2004; 52: 7846-56.

9. Ye J., Su J., Chen K., et al. Comparative investigation on chemical constituents of flower bud, stem and leaf of Lonicera japonica Thunb. by HPLC-DAD-ESI-MS/MS and GC-MS. J Anal Chem. 2014; 69 (8): 777-84.

10. Rop O., Reznicek V. Mlcek J., et al. Antioxidant and radical oxygen species scavenging activities of 12 cultivars of blue honeysuckle fruit. Hort Sci (Prague). 2011; 38 (2): 63-70.

11. Methods of analysis of minor biologically active substances of food. Edited by V.A. Tutelyan, K.I. Eller. Moscow: Dynastiya, 2010: 28-41. (in Russian)

12. Zhogova A.A., Perova I.B., Samylina I.A., Eller K.I., Ramenskaya G.V. Identification and quantitative determination of the main biologically active substances in motherwort herb by HPLC-mass spectrometry. Khimiko-farmatsevticheskiy zhurnal [Chemical-Pharmaceutical Journal]. 2014; 48 (7): 54-9. (in Russian)

13. Zhogova A.A., Samylina I.A., Eller K.I. Investigation of iridoids in buckbean (Menyanthes trifoliata) leaves. Farmatsiya (Pharmacy). 2013; (6): 17-20. (in Russian)

14. Perova I.B., Zhogova A.A., Polyakova A.V., Eller K.I., Ramenskaya G.V., Samylina I.A. Biologically active substances of cornelian cherry fruits (Cornus mas L.). Voprosy pitaniia [Problems of Nutrition]. 2014; 83 (5): 86-93. (in Russian)

15. Bondet V., Brand-Williams W., Berset C. Kinetics and mechanisms of antioxidant activity using the DPPH free radical method. Leb-ensm Wiss Technol. 1997; 30: 609-15.

16. Perova I.B. Investigation of the content and specific profile of medicinal plant anthocyanins: Diss. Moscow, 2015. (in Russian)

17. Lee J., Finn C.E. Anthocyanins and other polyphenolics in American elderberry (Sambucus canadensis) and European elderberry (S. nigra) cultivars. J Sci Food Agric. 2007; 87: 2665-75.

18. Malodobry M., Bieniasz M., Dziedzic E. Evaluation of the yield and some components in the fruit of blue honeysuckle (Lonicera caerulea var. edulis Turcz. Freyn.). Folia Horticulturae Ann. 2010; 22 (1): 45-50.

All articles in our journal are distributed under the Creative Commons Attribution 4.0 International License (CC BY 4.0 license)

SCImago Journal & Country Rank
Scopus CiteScore
CHIEF EDITOR
CHIEF EDITOR
Viktor A. Tutelyan
Full Member of the Russian Academy of Sciences, Doctor of Medical Sciences, Professor, Scientific Director of the Federal Research Centre of Nutrition, Biotechnology and Food Safety (Moscow, Russia)

Journals of «GEOTAR-Media»