The effect of carbon tetrachloride poisoning on the activity of digestive proteases in rats and correction of the violations with vegetable oils
AbstractThe results of the study of activity of digestive proteases (pepsin, trypsin, chymotrypsin) in homogenates of stomach, pancreas and duodenum in experimental animals have been presented. Rats were exposed to intoxication with carbon tetrachloride (subcutaneous administration of a 50% oil solution of ССl4 in thedose of 0,5 ml per 100 g body weight) for three days and then they were given analysed oils (black nut, walnut and flax oil) intragastrically by gavage at a dose of 0,2 ml per day within 23 days. Pepsin level in gastric mucosa homogenates and chymotrypsin activity in pancreatic homogenates were determined by method of N.P. Pyatnitskiy based on on the ability of enzymes to coagulate dairyacetate mixture, respectively, at 25 °C and 35 °C. Trypsin activity in homogenates of pancreatic was determined by method of Erlanger – Shaternikova colorimetrically. It has been established that intoxication with CCl4 decreased the synthesis of proteolytic enzymes of the stomach (by 51%) and pancreas (by 70–78%). Injections of analysed vegetable oils to animals contributed to the normalization of proteolytic enzymes synthesis. The conclusion that there are prospects of using the analysed vegetable oils containing large quantity of polyunsaturated fatty acids (ω-3 and ω-6) for the correction of detected biochemical abnormalities has been done.
Keywords:intoxication with carbon tetrachloride, rats, digestive proteases, vegetable oils