To the content
1 . 2021

Dihydroquercetin influence on clinical and biochemical blood parameters of pigs under conditions of stress load


In modern society, distress has become a widespread condition that negatively affects the functioning of all systems of the human organism. The study of biological mechanisms and changes in the organism under the influence of stress, as well as methods of their leveling, are relevant in medicine, animal science and veterinary medicine. Pigs are an excellent biological model that is closest to humans.

The aim of the research was to study the hematological and biochemical parameters of pigs out of and under stress, including against the background of daily consumption of the flavonoid dihydroquercetin (DHQ) with feed.

Material and methods. The research was conducted in the experimental yard of the L.K. Ernst Federal Science Center for Animal Husbandry on 3 groups of pigs [F2 hybrid (large whitexLandrace) x Duroc] with an initial body weight of 30-35 kg (n=27). Group 1K consisted of control animals not exposed to stress (n=9); group 2K - control animals subjected to simulated stress by the rearrangement of animals (n=9); group 3O - experimental animals subjected to simulated stress and fed throughout the entire experiment DHQ (32 mg per 1 kg of feed) (n=9). On days 0, 42, and 76, blood was collected from the animals and their hematological and biochemical parameters were studied using conventional methods.

Results. The positive effect of using DHQ in pigs’ nutrition on enhancing the oxidizing function of blood, metabolic intensity, and increasing the endurance of animals under stress conditions has been manifested in maintaining leukocyte level with a higher content of erythrocytes and hematocrit. In animals fed DHQ, alanine aminotransferase activity was lower than in animals not receiving DHQ. Stress led to a significant increase in lactate dehydrogenase activity in group 2K on the 46th day, which was not observed in animals treated with DHQ.

Conclusion. Long-term intake DHQ (up to 72 days inclusive) against the background of stress contributed to the preservation of blood values at the control level (without stress), within the physiological norm.

Keywords:stress, dihydroquercetin, clinical blood parameters, biochemical blood parameters, pigs

Funding. The research was carried out with the financial support of the Russian Science Foundation (project No. 19-16-00068).

Conflict of interests. Authors declare no clear or potential conflicts of interest.

For citation: Nekrasov R.V., Bogolyubova N.V., Semenova A.A., Nasonova V.V., Polishchuk E.K. Dihydroquercetin influence on clinical and biochemical blood parameters of pigs under conditions of stress load. Voprosy pitaniia [Problems of Nutrition]. 2021; 90 (1): 74-84. DOI: (in Russian)


1. Fomichev Yu.P., Nikanova L.A., Dorozhkin V.I., Torshkov A.A., Romanenko A.A., Es’kov E.K., et al. Dihydroquercetin and arabinogalactan – natural bioregulators in human and animal life, application in agriculture and food industry. Moscow: Nauchnaya biblioteka, 2017: 702 p. (in Russian)

2. Asmi K.S., Lakshmi T., Balusamy S.R., Parameswari R. Therapeutic aspects of taxifolin – an update. J Adv Pharm Educ Res. 2017; 7 (3): 187–9.

3. Sunil C., Xu B. An insight into the health-promoting effects of taxifolin (dihydroquercetin). Phytochemistry. 2019; 166: 112066. DOI:

4. Olifirova O.S., Kozka A.A. Modern comprehensive approach to the treatment of extensive long-term non-healing wounds. Sibirskoe meditsinskoe obozrenie [Siberian Medical Review]. 2017; 3 (105): 21–5. (in Russian)

5. Kharchenko Yu.A., Dmitriev V.N. Promising dietary Supplement with an antioxidant effect. Ratsional’noe pitanie, pishchevye dobavki i bioctumulyatory [Rational Nutrition, Food Additives and Biostimulators]. 2016; (3): 61–5. (in Russian)

6. Guseva T.B., Karan’yan O.M., Kulikovskaya T.S., Rassokha S.N., Radaeva I.A. Application of the natural antioxidant dihydroquercetin to increase the shelf life of canned milk. Pishchevaya promyshlennost’ [Food Industry]. 2017; (8): 54–6. (in Russian)

7. Nasonova V.V. Study of the possibility of using bioflavonoids in the production of meat products resistant to oxidative damage. Voprosy pitaniia [Problems of Nutrition]. 2016; 85 (S2): 207. (in Russian)

8. Guseva T.B., Karan’yan O.M., Kulikovskaya T.S. Application of the natural antioxidant dihydroquercetin to increase the shelf life and improve the biological value of products of the rosrezerva nomenclature. Innovatsionnye tekhnologii proizvodstva i khraneniya material’nykh tsennostey dlya gosudarstvennykh nuzhd [Innovative Technologies of Production and Storage of Material Values for State Needs]. 2017; 7 (7): 121–30. (in Russian)

9. Fomichev Yu.P., Nikanova L.A., Kleymenov R.V., Netecha Z.A. Application of dihydroquercetin and arabinogalactan in Piglet rearing. Veterinarnaya meditsina [Veterinary Medicine]. 2010; 5–6: 30–2. (in Russian)

10. Bizyuk L.A., Korolevich M.P. Antioxidant dihydroquercetin: clinical and pharmacological effectiveness and synthesis pathways. Lechebnoe delo [Medical Care]. 2013; 1 (29): 13-19. (in Russian)

11. Zhanataev A.K., Kulakova A.V., Nasonova V.V., Durnev A.D. In vivo study of dihydroquercetin genotoxicity. Bull Exp Biol Med. 2008; 145 (3): 338–40.

12. Zhigulina V.V. Biochemical response of the body to stress. Verkhnevolzhskiy meditsinskiy zhurnal [Verkhnevolzhsk Medical Journal]. 2014; 12 (4): 25–30. (in Russian)

13. Roux F., Saï, P., Deschamps, J.-Y. Xenotransfusion, past and present. Xenotransplantation. 2007; 14: 208–16. DOI:

14. Karkishchenko N.N. Fundamentals of biomodeling. Moscow, 2005: 608 p. (in Russian)

15. Bassols A., Costa C., Eckersall P.D., Osada J., Sabrià J., Tibau J. The pig as an animal model for human pathologies: a proteomics perspective. Proteomics Clin Appl. 2014; 8 (9–10): 715–31. DOI:

16. Nekrasov R.V., Golovin A.V., Makhayev E.A., et al. Norms needs dairy cattle and pigs in nutrients. In: L.K. Ernst Federal Science Center for Animal Husbandry. Moscow, 2018: 290 p. (in Russian)

17. Ventrella D., Dondi F., Barone F., Serafini F., Elmi A., Giunti M., et al. The biomedical piglet: establishing reference intervals for haematology and clinical chemistry parameters of two age groups with and without iron supplementation. BMC Vet. Res. 2017; 13 (1): 23. DOI:

18. Lozhkin A.P., Chernokhvostov Yu.V., Dvoinosov V.G., Panasyuk M.V., Zhdanov R.I. Influence of psychoemotional stress on the content of white blood cells and thrombodynamics in healthy volunteers. Kazanskiy meditsinskiy zhurnal [Kazan Medical Journal]. 2013; 94 (5): 718–22. (in Russian)

19. Martinez-Miro S., Tecles F., Ramon M., Escribano D., Hernandez F., Madrid J., et al. Causes, consequences and biomarkers of stress in swine: an update. BMC Vet Res. 2016; 12 (1). DOI:

20. Bogolyubova N.V., Chabaev M.G., Fomichev Y.P., Tsis E.Y., Semenova A.A., Nekrasov R. Ways to reduce adverse effects of stress in pigs using nutritional factors. Ukrainian J Ecol. 2019; 9: 239–45.

21. Polishchuk S.A., Molyanova G.V. Dynamics of morphological parameters of dog blood when adding dihydroquercetin. Izvestiya Samarskoy gosudarstvennoy sel’skokhozyaystvennoy akademii [Proceedings of the Samara State Agricultural Academy]. 2016; (2): 60–3. DOI: (in Russian)

22. Chernukha I.M., Kotenkova E.A., Vasilevskaya E.R., Ivankin A.N., Lisitsyn A.B., Fedulova L.V. The study of biological effects of different geographical origin goji berries in rats with alimentary hypercholesterolemia. Voprosy pitaniia [Problems of Nutrition]. 2020; 89 (1): 37–45. DOI: (in Russian)

23. Britov A.N., Aparina T.V. the Role of «Capilar» (dihydroquercetin) in the correction of hemadynamic and metabolic disorders in patients with atherosclerosis and arterial hypertension. Regionarnoe krovoobrashchenie i mikritsirkulyatsiya [Regional Blood Circulation and Microcirculation]. 2006; (2): 46–53. (in Russian)

24. Peng J., Li Q., Li K., Zhu L., Lin X., Lin X., et al. Quercetin improves glucose and lipid metabolism of diabetic rats: involvement of Akt signaling and SIRT1. J Diabetes Res. 2017; 2017: 3417306. DOI:

25. Li Y., Yao J., Han C., Yang J., Chaudhry M., Wang S., et al. Quercetin, inflammation and immunity. Nutrients. 2016; 8: 167. DOI:

26. Martinez, G., Mijares T., Michael R., De Sanctis, J. Effects of flavonoids and its derivatives on immune cell responses. Recent Pat Inflamm Allergy Drug Discov. 2019; 13 (2): 84–104. DOI:

SCImago Journal & Country Rank
Viktor A. Tutelyan
Full Member of the Russian Academy of Sciences, Doctor of Medical Sciences, Professor, Scientific Director of the Federal Research Centre of Nutrition, Biotechnology and Food Safety (Moscow, Russia)
Medicine today

II Конференция онкологов Московской области Москва, 4 июня 2021 года Подмосковье в настоящее время - это регион, переживающий бурный прирост населения и активную урбанизацию. Эти процессы требуют изменений в организации оказания медицинской помощи, в первую очередь,...

Приглашаем вас принять активное участие в работе XII Международной конференции "Актуальные аспекты экстракорпорального очищения крови в интенсивной терапии". Мероприятие пройдет 28-29 мая 2021 года в онлайн-формате. Конференция организована при поддержке Министерства...

Конференция РАРЧ "Репродуктивное здоровье и эпидемия COVID-19: год спустя" 8 июня 2021 г., онлайн Коронавирусная инфекция внесла существенные коррективы в работу специалистов в области репродуктивного здоровья. За прошедший год мы преодолели множество совершенно необычных...

Journals of «GEOTAR-Media»