Instant drinks based on extracts of Japan sea brown algae and fruit and berry juices as functional products

Abstract

Nowadays, there is a need to create functional drinks that not only normalize the water-electrolyte balance, but also correct and optimize the chemical structure of the diet. The basis for the production of dry drinks can be fruit and berry juices, extracts of herbal raw materials, including algae, etc.

The aim of the study was to develop dry drinks based on dry extracts of brown algae (Costaria costata and Undaria pinnatifida) and concentrated fruit and berry juices and to evaluate the content of biologically active substances in them, as well as the antioxidant properties of the resulting drinks.

Material and methods. Brown algae of the Far Eastern region Costaria costata and Undaria pinnatifida were used as objects, from which dry hydrothermal extracts were obtained, as well as dry drinks based on these extracts (21–26%) and concentrated fruit and berry juices of black currant and blueberry (31–37%). The content of iodine was determined by titrimetric method, fucoidan, the sum of phenolic compounds, flavonoids, catechins – by spectrophotometric method, vitamin C – titrimetrically, anthocyanins – by pH-differential spectrophotometry. Identification of phenolic compounds was carried out by HPLC. The antiradical properties of beverages were evaluated by the ability to interact with the stable 2.2-diphenyl-1-picrylhydrazyl free radical in vitro spectrophotometrically.

Results. Dry hydrothermal extracts of C. costata and U. pinnatifida brown algae are characterized by a high content of bioactive substances, the main of which are fucoidan and iodine. The fucoidan content in C. costata extract was 1.7 g/100 g, in U. pinnatifida extract – 0.5 g/100 g; the iodine content was 0.0036 and 0.0028 g/100 g, respectively. The content of phenolic compounds was at least 205 mg of tannic acid per 1 g, the main compounds were syringic acid and epicatechin, salicylic and coumaric acids, as well as chlorogenic, caffeic, 2.5-dihydroxybenzoic, ferulic acid and gallates of epigallocatechin and epicatechin. The developed drinks based on dry extracts of C. costata and U. pinnatifida brown algae (21–26%) and concentrated blueberry and blackcurrant juices (31–37%) (the rest is powdered sugar) are a food system enriched with bioactive substances. The content per 1 serving of drinks (10 g per 200 ml) of iodine was high and amounted to 70–75 mcg, phenolic compounds – about 250 mg, vitamin C level was maximum in a drink with black currant (41–44 mg), fucoidan content ranged from 79 to 84 mg. The resulting dry drinks satisfy the daily physiological requirement in iodine by at least 40%, in vitamin C – by at least 30% when consuming 1 serving. The radical binding activity of all the studied beverages was quite high and amounted to 91.1–96.5%. The drink with blueberry juice and C. costata extract showed maximum antiradical properties.

Conclusion. The developed dry soft drinks based on dry extracts of C. costata and U. pinnatifida brown algae and concentrated juices of black currant or blueberry contain a wide range of bioactive compounds. They can be attributed to functional products due to the high degree of satisfaction of the daily physiological requirement of the human body in vitamin C and iodine and are characterized by high antiradical properties.

Keywords:dry drinks; brown algae Costaria costata and Undaria pinnatifida; biologically active substances; fucoidan; iodine; black currant and blueberry juices; antiradical activity

Funding. The research was supported by the grant of the President of Russian Federation MK-4715.2021.4.

Conflict of interest. The authors declare the absence of a conflict of interest.

Contribution. The authors declare an equal contribution in the preparation of the article.

For citation: Tabakaev A.V., Tabakaeva O.V. Instant drinks based on extracts of Japan sea brown algae and fruit and berry juices as functional products. Voprosy pitaniia [Problems of Nutrition]. 2022; 91 (4): 107–14. DOI: https://doi.org/10.33029/0042-8833-2022-91-4-107-114 (in Russian)

References

1. Zuev E.T. Functional drinks: their place in the concept of functional nutrition. Pishchevaya promyshlennost’ [Food Industry]. 2004; (7): 90–4. (in Russian)

2. Cao L., Lee S.G., Lim K.T., Kim H.R. Potential anti-aging substances derived from seaweeds. Mar Drugs. 2020; 18 (11): 564–70. DOI: https://doi.org/10.3390/md18110564

3. Gerasimenko N.I., Martyyas E.A., Busarova N.G. Composition of lipids and biological activity of lipids and photosynthetic pigments from algae of the families Laminariaceae and Alariaceae. Chem Nat Compd. 2012; 48: 737–41. DOI: https://doi.org/10.1007/s10600-012-0371-5

4. Sukhoveeva M.V., Podkorytova A.V. Commercial algae and grasses of the seas of the Far East: biology, distribution, reserves, processing technology TINRO-center. Vladivostok, 2006: 243 p. (in Russian)

5. Tabakaeva O.V., Tabakaev A.V. Biologically active agents of potential trade brown seaweed of the Far East Region. Voprosy pitaniia [Problems of Nutrition]. 2016; 85 (3): 126–32. DOI: https://doi.org/10.24411/0042-8833-2016-00044 (in Russian)

6. Bakin I.A., Mustafina A.S., Lunin P.N. The study of the black currant berry chemical composition in the processing. Vestnik Krasnoyarskogo gosudarstvennogo agrarnogo universiteta [Bulletin of Krasnoyarsk State Agrarian University]. 2015; (6): 159–62. (in Russian)

7. Velichko N.A., Berikashvili Z.N. The study of the chemical composition of berries of blueberry and common development of formulations of drinks on its basis. Vestnik Krasnoyarskogo gosudarstvennogo agrarnogo universiteta [Bulletin of Krasnoyarsk State Agrarian University]. 2016; (7): 126–31. (in Russian)

8. Sirenko L.A., Sakevich A.I., Osipov L.F., Lukina L.F., Kuz’menko M.I., Kozitskaya V.N. Methods of physiological and biochemical research of algae in hydrobiological practice. Kiev: Naukova dumka, 1975: 253 р. (in Russian)

9. Smirnova G.R., Klochkova N.I. Polysaccharide composition of some brown algae of Kamchatka. Bioorganicheskaya khimiya [Bioorganic Chemistry. 2001; 27 (6): 444–8. (in Russian)

10. Sapozhnikov D.I. Pigments of plastids of green plants and methods of their research. Moscow: Nauka, 1964: 129 p. (in Russian)

11. Calado J., Albertão P.A., de Oliveira E.A., Letra M.H.S. Flavonoid contents and antioxidant activity in fruit, vegetables and other types of food. Agric Sci. 2015; 6: 426–35. DOI: https://doi.org/10.4236/as.2015.64042

12. FFS 1.5.3.0008.15. The determination of tannins in herbal drugs and medicinal plant preparations. URL: http://193.232.7.120/feml/clinical_ref/pharmacopoeia_2/HTML/#417/z (in Russian)

13. Molyneux P. The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin J Sci Technol., 2004; 26 (2): 2119.

14. Yoo H.J., You D.-J., Lee K.-W. Characterization and immunomodulatory effects of high molecular weight fucoidan fraction from the sporophyll of Undaria pinnatifida in cyclophosphamide-induced immunosuppressed mice. Mar Drugs. 2019; 17 (8): 447. DOI: https://doi.org/10.3390/md17080447

15. Saetan U., Nontasak P., Palasin K., Saelim H., Wonglapsuwan M., Mayakun J. et al. Potential health benefits of fucoidan from the brown seaweeds Sargassum plagiophyllum and Sargassum polycystum. J Appl Phycol. 2021; 33 (5): 3357–64. DOI: https://doi.org/10.1007/S10811-021-02491-3

16. Kordjazi M., Etemadian Y., Shabanpour B., Pourashouri P. Chemical composition antioxidant and antimicrobial activities of fucoidan extracted from two species of brown seaweeds (Sargassum ilicifolium and S.angustifolium) around Qeshm Island. Iran J Fish Sci. 2019. Vol. 18, N 3. P. 457–75. DOI: https://doi.org/10.22092/IJFS.2018.115491

17. Khil’chenko S.R., Zaporozhets T.S., Zvyagintseva T.N., Shevchenko N.M., Besednova N.N. Fucoidans from brown algae: the influence of molecular architecture features on functional activity. Antibiotiki i khimioterapiya [Antibiotics and Chemotherapy]. 2018; 63 (9–10): 69–79. (in Russian)

18. Zvyagintseva T.N., Usoltseva R.V., Shevchenko N.M., Surits V.V., Imbs T.I., Malyarenko O.S., et al. Structural diversity of fucoidans and their radioprotective effect. Carbohydr Polym. 2021; 273: 118551. DOI: https://doi.org/10.1016/j.carbpol.2021.118551

19. Besednova N.N., Zaporozhets T.S., Kuznetsova T.A., Makarenkova I.D., Kryzhanovsky S.P., Fedyanina L.N., et al. Extracts and marine algae polysaccharides in therapy and prevention of inflammatory diseases of the intestine. Mar Drugs. 2020. Vol. 18, N 6. Р. 289. DOI: https://doi.org/10.3390/md18060289

20. Besednova N.N, Andryukov B.G., Zaporozhets T.S., Kryzhanovsky S.P., Fedyanina L.N., Kuznetsova T.A., et al. Antiviral effects of polyphenols from marine algae. Biomedicines. 2021. Vol. 9, N 2. P. 1–23. DOI: https://doi.org/10.3390/biomedicines9020200

21. Li J., Guo C., Wu J. Fucoidan: biological activity in liver diseases. Am J Chin Med. 2020; 48 (7): 1617–32. DOI: https://doi.org/10.1142/S0192415X20500809

22. Skal’naya M.G. Iodine: the biological role and significance for medical practice. Mikroelementy v meditsine [Trace Elements in Medicine]. 2018; 19 (2): 3–11. DOI: https://doi.org/10.19112/2413-6174-2018-19-2-3-11 (in Russian)

23. Tutelyan V.A., Lashneva N.V. Biological active substanse of plant origin. Phenolic acids: occurrence, dietary sourses biovailability. Voprosy pitaniia [Problems of Nutrition]. 2008; 77 (1): 4–19. (in Russian)

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CHIEF EDITOR
CHIEF EDITOR
Viktor A. Tutelyan
Full Member of the Russian Academy of Sciences, Doctor of Medical Sciences, Professor, Scientific Director of the Federal Research Centre of Nutrition, Biotechnology and Food Safety (Moscow, Russia)

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