Indicators of vitamins safety in experimental alimentary
hyperlipidemia in rodents
Rats and mice of different strains are used as a model of metabolic disturbances, caused by the consumption of diets with unbalanced content of macro-nutrients (fat, carbohydrate), as well as having elevated cholesterol quota. The aim of this study was to determine the magnitude and direction in change of vitamins status indices produced in rats and mice with experimental-mental hyperlipidemia, developing under consumption of high fat diet (HFD), fructose (Fr) and cholesterol (Cho). The experiment was conducted on 48 female growing Wistar rats with initial body weight 122 ±12 g, and 48 female growing C57Black/6 mice with initial body weight 18±1 g, which were divided into 12 groups of 8 animals per group. Within 63 days the rats and mice of the first (control) group received a balanced semi-synthetic (BD), 2nd groups - HFD with 30% of the total fat by weight of dry feed, 3rd groups - BD and Fr solution instead of water, 4th groups - HFD+Fr, 5th groups - BD supplemented with 0.5% Cho by weight of dry food, 6th groups - the same ration and Fr. After removal of animals from the experiment there were determined the content of vitamin A (retinol and retinol palmitate) and E (α-tocopherol) in blood plasma and liver by HPLC, 25-hydroxycholecalciferol [25(OH)D] in blood plasma by HPLC-MS, vitamins B1, B2 and oxidized NAD coenzymes in liver by fluorimetric methods. Consumption of HFD resulted in marked increase in the concentration of vitamin A by 32% and by 45% in rat blood plasma and in the mice liver respectively, elevation of vitamin E level by 46% in the rat liver. Unlike rats, vitamin E in the liver of mice treated with HFD was lower by 32% compared with the control. Cho additive resulted in increased vitamin E accumulation in rat and mice liver (α-tocopherol level was 2.5 и 1.5 fold higher than in control respectively). Convincing evidence wasn't revealed of the impact of the additional Fr on vitamins A and E safety in rats and mice. Consumption of Fr on background of HFD in rats significantly reduced the level of 25(OH)D compared with HFD without Fr. Fr reception in combination with the addition of Cho significantly reduced stores of vitamin A and increased - of vitamin E in the liver of rats and mice. 25(OH)D level for this type of diet was significantly reduced. Cho consumption in rats significantly decreased the content of NAD+NADP in the liver by 12%; the introduction of fructose into the diet neutralized this impact. Feeding rats with HFD resulted in a significant improvement, and uptake of Cho in reduce of vitamin B2 levels in the liver by 12.8 and 28%, respectively. Fr partially neutralized these effects. Thus, changes in the ratio of macronutrients and Cho in the diet of rats and mice may lead to a partially species-specific vitamin sufficiency variations, including in some cases the development of functional deficiency of vitamins А, B2, D and NAD coenzymes.
Antibiotic resistance of Campylobacter jejuni strains isolated from food products
Campylobacter jejuni is a leading member of the genus Campylobacter which cause up to 90% of laboratory confirmed cases of campylobacteriosis. The most important characteristic defining the biological features of C. jejuni is their sensitivity to antibiotics. Agricultural intensification, expansion of the range of the used disinfectants and antiseptics, uncontrolled use of antibiotics in animal production is increasingly leading to the selection of the most resistant forms of Campylobacter with antibiotic resistance and multiple virulence factors. The study of antibiotic resistance of C. jejuni isolated from food and environment need for the development of new approaches for laboratory diagnosis of campylobacteriosis and confirmation of the role of food path of transmission, for creation the system of preventive measures to reduce the risk of contamination of food by Campylobacter spp. in Russia. The aim of this study was to investigate the phenotypic profiles of antibiotic resistance of Campylobacter spp. isolated from poultry and the environment in the poultry processing industry. In the analysis of 110 samples of raw poultry products and swabs from surfaces of the equipment 55 strains of the genus Campylobacter were selected, including 46 strains of C. jejuni. For study sensitivity of these strains to 15 antimicrobials (8 classes) disk diffusion assays were done using the EUCAST protocol. The following antibiotics were used: nalidixic acid, ciprofloxacin, erythromycin, gentamicin, amikacin, kanamycin, tetracycline, oxytetracycline, doxycycline, clindamycin, lincomycin, ampicillin, chloramphenicol, florfenicol, cefotaxime. All C. jejuni strains were resistant to cephalothin, which confirms their belonging to this species. 89% of the strains were insensitive to nalidixic acid, which indicates the reduction of informativeness of this test, traditionally used in the standard scheme of species identification of Саmpylobacter spp. Most of the investigated isolates were resistant to ciprofloxacin (96.3%) and tetracycline (88.6%), 34% of strains had resistance to erythromycin; 40% of tested C. jejuni were multi-resistant to four or more antibiotics. The data indicate a high prevalence of antibiotic-resistant strains among campylobacteria, contaminated poultry products during the processing of raw materials.
The study of rs11801197 polymorphism of the calcitonin receptor gene (CALCR) in Moscow women and children with different level of bone strength
The frequency of the polymorphism rs11801197 of calcitonin receptor gene (CALCR) was studied by real-time PCR in 422 Moscow women and children, including pregnant women (n=96), lactating (n=29) and non-pregnant women (n=28) and children (n=269) of preschool (2-6 years, n=76) and school age (7-16 years, n=193) with different levels of bone strength (BS) as determined by ultrasound densitometry. It was found that the decrease in the value of the BS (Z-score<-1) was observed in 60% of women, 54% of preschool children and 48% of school children. In the cohort studied the predominant genotype of rs11801197 polymorphism of CALCR gene was CT (38%), the frequency of the genotypes CC and TT - 31%, C and T allele - 50%. There was statistically significant association of BS reduction risk with a C allele of rs11801197polymorphism of CALCR gene in all examined women (QR=2.034, p=0.02). A positive but not statistically significant association of BS reduction risk with C allele of polymorphism in non-pregnant andpregnant women was found (OR=6.905, p=0.09 and OR=1.902, p=0.09 respectively). The same tendency was observed in preschool children (OR= 1.880, p=0.104). In school-aged children C allele was not associated with the risk of BS reduction (OR=0.866, p=0.595). Thus, the allele C is the risk allele of BS reduction. The frequency of CC genotype in Moscow women is much higher than that in women in Europe and in women of North-West region of Russian Federation. Women in the Moscow region - the carriers of rs11801197 polymorphism of CALR - gene need in personalized support of their bone health.
Evaluation of the cytolytic effect of tonic with ginseng extract
Experimental evaluation of the cytolytic effect of prolonged use of tonic drink with ginseng extract by determining the activity of enzymes, markers of cytolysis (alanine aminotransferase, aspartate aminotransferase, creatine kinase and lactate dehydrogenase (LDH)) has been carried out. The study was performed on male rats Wistar (n=15, body weight of 461±23 g), to which tonic drink (containing 46 mg/L panaxosides) was administered in the dose 15 mg/kg body weight using a pharyngeal tube. The introduction was carried out three times daily for 21 days. Then the activity of enzymes - markers of cytolysis was determined in bloodplasma. Three-factor mathematical models predicting changes in LDH activity under prolonged intake of tonic drink with ginseng extract were constructed by regression analysis. The models were characterized by close ties of effective and factorial signs under reliability level of 95%, were adequate by the Fisher test, and the coefficients were significant by Student's test. It was found that a daily intake three-time intake of tonic drink with ginseng extract for 21 days didn't lead to significant changes in the activity of enzymes, markers of cytolysis. This coincided with the forecast estimate of cytolysis using three -factor mathematical models. However, the proposed mathematical models allowed to reveal that changing in the dosage and concentration of the beverage may increase LDH activity corresponding to cytolysis.
Assessment of the relationship of inflammation markers, adipokines and parameters of lipid metabolism in patients with overweight and obesity
Objective - to assess the changes and the relationship of inflammation markers, adipokine levels and lipid metabolism in overweight and obesity, with signs of the metabolic syndrome. The study involved 62 women aged from 25 to 50 years. Group 1 included women (n=20) with a BMI<27.0, with no signs of metabolic syndrome, group 2 (n=20) -with a BMI>27.0, who were overweight or obese, with no signs of metabolic syndrome, 3 group (n=22) with a BMI>27.0, having overweight or obesity and the presence of any signs of metabolic syndrome. We determined relative and absolute lymphocyte counts and neutrophil granulocytes in the blood, lipid spectrum, blood serum levels of apolipoprotein A-1 (ApoA-1), and apolipoprotein B (apoB), leptin, adiponectin, interleukin-1β (IL-1β), interleukin 6 (IL-6), C-reactive protein (CRP). It was noted (p≤0.001) an increase of CRP in the blood of obese women, regardless of the presence of the metabolic syndrome: its concentration in groups 2 and 3 reached 6.69±3.03 and 7.30±3.08 mg/l, respectively, against 2.39±1.66 mg/l in group 1. Increasing BMI was accompanied by (p≤0.01) increase in erythrocyte sedimentation rate (8.1±5.0; 14.3±8.7; 13.9±8.5 mm per hour, respectively, in groups 1, 2 and 3). In women from groups 1 and 3 an increase in triglycerides, accompanied by a decrease in HDL (r=-0.44; p<0.025 and r=-0,57; p<0.001, respectively), and increase of LDL cholesterol (r=1.0;p<0.001) has been shown. The maximum number of positive correlations between indicators of lipid profile and inflammation markers was found in group 1, the minimum in group 3. This analysis showed the presence of chronic inflammation, which was accompanied by various disorders of lipid metabolism, even in the absence of clinical signs of pathology, the severity of which increased with increasing BMI and the development of metabolic syndrome.
Diet optimization in patients with obesity and diastolic heart failure based on the assessment of indicators of metabolism during physical exertion
The objective of the work was to examine the performance of basal metabolism and body composition in obese patients with diastolic heart failure (DHF) to identify optimal needs of these patients in the nutrients and energy. We studied 222 patients with obesity, that were divided into four groups depending on the degree of obesity and the presence or absence of DHF with preserved systolic function of the left ventricle (2 groups of obese patients with an initial degree I-II, 2 groups - a pronounced degree of obesity III). Basal metabolism by indirect respiratory calorimetry, physical activity at home and cardiorespiratory exercise testing were evaluated in these groups of patients. It has been shown that patients with obesity and secondary DHF have significantly lower physical activity during the day, resulting in the reduction of the overall length of the active time of day to 18.5-26.9%, and in the decrease of the proportion of time performing heavy and moderate work to 27.6-80.3%. Anaerobic threshold in obese patients I-II degree was 62.9% of maximum oxygen consumption (VO2pik) (rate is more than 60%), in patients with obesity III degree - 77.6% of VO2pik. Alveolar gas exchange also was not broken - the CO2 ventilation equivalent was equal respectively 23.4±2.8 and 28.9±1.5 (rate is less than 32). Thus, cardiopulmonary exercise testing have demonstrated that cardiopulmonary causes of dyspnea in obese patients without DHF were absent; and the reason for the decrease of exercise tolerance in patients with obesity III degree was the detraining. The reason for inactivity in patients with obesity and DHF was a marked reduction in exercise capacity due to violations of alveolar ventilation and reducing aerobic capacity of muscles. It was found that accustomed physical activity of patients at home didn't allow patients with obesity and DHF to carry out effective fat oxidation, due to the rapid achievement of the anaerobic threshold. It has been suggested that this effect may be the cause of metabolic progression of obesity in patients with DHF. It was found that patients with obesity and secondary DHF were characterized by a more pronounced oxidation of the protein, compared with similar patients without DHF, which made actual the muscle mass patronage under diet therapy management of patients. The optimum performance of the chemical composition and energy value of the diet have been calculated forpatients with obesity of varying severity in conjunction with SDS.
Cardioprotective effect of new functional food containing salmon oil with motherwort oil extract
Combination of fish oil with herbal oil extract can extend functional properties of the products. A new functional food of salmon oil with oil extract of motherwort (8:2) was developed and studied. Qualitative profiling was evidenced about presence of flavonoids and iridoids. Quantification of vitamins A (210±3 IU/g), Е (2.8±0.1 mg/g), D3 (12.2±0.4 IU/g) and PUFA (43.3±0.4%, incl. 9.4±0.1% of omega-3 and 33.9±0.1% of omega-6) was done by modern methods. The developed product was found nontoxic (LD50>15 000 mg/kg b.w., intragastriсally, LD50>3000 mg/kg b.w., intraperitoneal). The 14 days intragastric administration of product to the rats (initial b.w. 200-250 g) at the doses of 2340 and 1170 mg/kg b.w. significantly increased the left ventricular pressure after ischemia comparing with control, and normalized the contraction and relaxation of the left ventricle. Biochemical data confirmed the decrease of aspartate amino transferase and creatine kinase activity in rats treated at all experimental doses. Application of fish oil combination with motherwort oil extract is promising for development of new functional foods with cardio protective properties.
Influence of meat semi-prepared foods produced with an addition of water having a reduced deuterium content on the indicators of the laboratory animals with the model of alloxan diabetes
The paper presents the results of the study on the influence of water with a reduced deuterium content and the meat semi-prepared foods produced with its addition on the indicators of the laboratory animals with the model of alloxan diabetes. The effect of low concentrations of deuterium on the body weight dynamics was shown as well as the manifestation of glucosuria and ketonuria, and clinico-biological indicators of the animal's blood. The experiment was carried out during 42 days on male rats of Wistar stock, which were divided into 4 groups - 2 experimental, control (n=10) and intact (n=6), and consisted of three stages: adaptational, the stage of modeling of alloxan diabetes (a single intraperitoneal injection of alloxan monohydrate - 12 mg/100 g body weight) and dietic therapeutic. The animals of the experimental groups consumed water with a reduced deuterium content and standard vivarium diet or the meat minced semi-prepared products produced with its addition and cooked to culinary readiness. The control group consumed tap water and standard vivarium diet. In animals of the 1st group, which consumed the meat product, glucosuria persisted up to the 28th day (the 11th day after administration of alloxan), ketonuria up to the 34th day (the 17th day after modeling). In rats of the 2nd group, which consumed water with the decreased deuterium content, glucose in urea was not detected already on the 7th day after administration of alloxan, ketones on the 17th day after modeling of the disease, respectively. In animals of the control group, glucosuria and ketonuria persisted up to the end of the experiment. As a result of the investigations, the positive effect of water with a reduced deuterium content and the meat food produced with its use was established. It was manifested in a decrease of glucose level in the urea of the animals from the experimental groups compared to the control group on the 5th day of disease modeling and normalization on the 17th day of modeling. This experimentally demonstrated the possibility to use deuterium-depleted water in a meat product composition intended for nutrition of people with an impairment of carbohydrate metabolism for correction of metabolic processes.
The biological activity of the composition with glucosamine in the diet of rats in the modeling of cartilage and connective tissue damage
The results of experimental researches of the biological activity and physiological effectiveness of glucosamine consumption, including in the combination with glucose, gelatin and camellia, for rats with damaged cartilage and connective tissue have been presented. Animals of the control group (n= 12) received a standard diet. Rats from the 2nd and the 3rd groups in addition to the basic diet during 5 days before and the 48 hours on experiment orally consumed the glucosamine sulphate separately prepared at a dose of 10.98 mg/kg of body weight and respectively in dose 900 mg/kg of nutritional composition (44.6% of treated drinking water, 50% of glucose, 4% of gelatin, 1.22% of glucosamine sulfate, 0.15 and 0.03% of xanthan and guar gums). After modeling acute inflammatory edema, induced by administration of hind paw 0.1 ml of a 10% suspension of kaolin, the amounts of rats claws were measured in each of the tested groups. The results indicated much lesser extent of damage in case of glucosamine sulphate consumption, both independently and in combination with glucose and gums, while comparing with the control group. For the animals of the 2nd and the 3rd groups the maximal volume of rats claws occurred earlier (in the range of 4 hr). Also it was 20.2 and 21.5% less compared with the same index of control animals, which had maximal volume of claws almost during the 5th hour after the injury. It was also found that the animals treated with glucosamine sulfate, both separately and in combination with glucose and gums, restored after simulation of acute inflammatory edema more quickly - the volume of tabs on 48 h after injury was 57 and 64% less comparing with the same index in the control group, respectively. Basing on the presented data, it was confirmed that the glucosamine in a combination with glucose, gums and gelatin had positive effect on metabolic processes in the tissues, joints and there was a rationality of this biologically active nutrient's usage in the production of confectionery products.
Development of liposomal form of polyunsaturated fatty acid concentrate: ways of using in production of functional foods
The research is aimed at obtaining a concentrate of polyunsaturated fatty acids (PUFA) in liposomal form and developing possible ways of PUFA application in manufacturing functional food products. The PUFA concentrate was obtained from the fat of the Baikal seal by method of complexing with urea. We prepared a liposomal form of PUFA concentrate by evaporating organic solvent from the solution of lipids to form a thin lipid film with addition of buffer solution and further extrusion of the obtained emulsion. We defined the size of liposomal particles by method of turbidimetry. We identified the fatty acid composition of the samples by gas-liquid chromatography using a chromatographymass spectrometer. Biomedical research was conducted on 30 adult male Wistar rats with initial weight of 130-150 g. We induced atherogenic experimental hyperlipemia in rats by adding 5% cholesterol, 0.3% of 6-methyl-2-thiouracil, 1% cholic acid and 5% of lard to their normal diet for 21 days. We used an automatic biochemical analyzer to define total cholesterol (TC), triglycerides (TG), high density lipoprotein (HDL), low density lipoprotein (LDL), very low density lipoproteins (VLDL) in blood serum. As a result of the experiment, we obtained a concentrate from the seal fat with content of essential PUFA 12.2% of total fatty acids in which the ratio of ω-6:ω-3 was 1.3:1. We obtained the liposomal form of the PUFA concentrate, with the average size of the liposomes ranging from 144 to 158 nm. We have established that the blood serum of the animals with induced hyperlipidemia, which orally took the liposomal form of PUFA concentrate for 14 days in a dose of 20 mg/kg body weight, showed the decrease of total cholesterol content of LDL and VLDL, while HDL increased to the parameters in intact animals. This indicated a normalization of the lipid profile in rats and the lipid-lowering effect of the concentrate. Also, we have studied the possibility of enriching wheat bread with essential fatty acids by replacing some part of the water with the liposomal form of PUFA concentrate. The use of this additive increased the output volume of bread by 14%, intensified the crust color and didn't affect the organoleptic indicators of bread quality. Fatty acid analysis of the lipids in bread showed that after adding 3 g of PUFA concentrate in liposome form per 100 g of flour increased the content of ω-3 PUFA by 3.2 fold (191 mg per 100 g of bread), with the ratio of ω-6:ω-3 PUFA being 5.8:1. Thus, bread enriched with PUFA concentrate in liposome form can be positioned as a functional food product that contains ω-3 PUFA in an amount of 19.1% of the recommended daily intake in 100 g of final product and also as a product with optimal PUFA ratio of ω-6:ω-3.
The effectiveness of com bined treatment with simbiotiks and antioxidants in patients with periodontal disease and metabolic syndrome
The article discusses the pathophysiological mechanisms of the relationship between inflammatory periodontal diseases and metabolic syndrome (MS). Clinical and biochemical assessment of the dynamics of indicators of lipid blood profile, carbohydrate metabolism and oxidative stress has been conducted in patients with periodontal disease and MS. We examined and treated 73 patients aged from 35 to 55 years with clinical and biochemical features of the MS (body mass index (BMI) >25 kg/m2, abdominal type of obesity, impaired glucose tolerance and atherogenic dyslipidemia). According to the data obtained, the inclusion of probiotics and antioxidant in the complex therapy on the background of the reduced low-carbohydrate diet and increased physical activity in MS patients contributed to the correction of atherogenic blood plasma indexes, normalization of carbohydrate metabolism, cupping effects of oxidative stress, improvement of reparative processes in periodontal tissues, more pronounced than in patients receiving standard treatment (control). After treatment at 24 weeks BMI decrease by 8.6% (from 32.7±0.8 to 29.9±0.6 kg/m2) mainly due to the reduction in fat mass (by 23%) has been observed in the whole group of patients with MS. The HOMA IR index decreased in patients of the main group by 31.8% while in the control group - only by 8.5% (p<0.001), fasting immunoreactive insulin level - by 13 vs 4.5% (p<0.001), insulin C-peptide level - by 36.8 vs 21.6% (p<0.01), glycated hemoglobin - by 13.0% versus 4.7% (p<0.01), malondialdehyde - by 47.8 vs 25.8% (p<0.01). Levels of total cholesterol, triglycerides and low-density lipoproteins decreased in patients of the main group by 13.0; 30.8 and 19.0%, respectively, while in the control group the decrease was lower and amounted to 4.7; 25.0 and 14.6% (p<0.05-0.01).
Evaluation of preventive effectiveness of food products by community-acquired pneumonia with unknown causative agent
The aim of the study is to evaluate a preventive effectiveness of food products with elevated content of biologically active substances by community-acquired pneumonia with unknown causative agent. The authors studied two groups of youngsters (18 years old) during period of adaptation to conditions of organized group: with normal nutritional status [with body mass index (BMI) = 19.5-22.9 kg/m2] and decreased one (with BMI = 18.5-19.4 kg/m2). Tumor necrosis factor (TNFa) and interleukin 1α (1L-1β) levels in blood serum were criteria of degree of inflammatory process. Functional activity of phagocytes was also evaluated with the use of nitroblue tetrasol test (NTT-test). To prevent pneumonia natural concentrated food product made of vegetable raw materials (3.0 g per day) by cryogen technology was added in diet of persons with normal nutritional status during two periods (15 days) with an interval (30 days). But persons with decreased nutritional status got the product made of protein-vegetable raw materials during 15 days only and additionally product made of protein-vegetable raw materials by cryogen technology one time per day (35.0 g) during 15 days. The persons with normal nutritional status were divided in three group: persons (480 men) in the first group got the product only, persons (100 men) of the second group underwent vaccination with polysaccharide polyvalent «Pneumo-23» vaccine only, persons (316 men) of the third group underwent vaccination in combination with the product intake. The persons (231 men) with decreased nutritional status composed one group and got the food product only. Control group consisted of 263 men with normal nutritional status. The authors evaluated incidence rates during three months of observation and duration of in-patient treatment. The results were compared with data obtained in baseline period (the same time periods during three years before the study). The authors received data on the decrease of a reserve ability of phagocytes; it provided evidence about an initial deficiency of immune protection factors in persons with decreased nutritional status. In adaptation period, this phenomenon was observed in persons with normal nutritional status too. The including of protein-vegetable product in diet promoted the increase of bactericidal function of neutrophils and body weight among youngsters with decreased nutritional status; but including of vegetable product kept bactericidal function of neutrophils on normal level. In adaptation period, TNFa levels increased in both groups by 21.0 and 35.1%, respectively; 1L-1β level did not significantly change in group with normal nutritional status, but increased 2.25 fold in persons with decreased nutritional status. The levels of studied cytokins decreased to baseline values in 30 days after the product intake. To this time, TNFa increased 1.8 fold and 1L-1β increased 2.86 times in control group. A preventive effectiveness of the especially created products in nidus with unknown causative agent was confirmed by the decrease of incidence rate (82.4-78.4%) in persons with normal and lower nutritional status that coincided with effectiveness of a vaccinal prevention only. The food product with antioxidant properties potentiated a vaccinal prevention. The positive effect consisted in lack of fatal outcomes and outbreaks of the disease; mean bed-day decreased on 3.5 days; predominance of mild form of the disease in clinical picture in comparison with same parameters during basic period of observation.
Efficiency of polyphenol-rich grape products for the prevention of metabolic disorders in the experiment
The article presents the results of study of the effect of polyphenol-rich materials obtained from grapes on the clinical symptoms, activity of non-specific proteases, and parameters of lipid peroxidation (LPO) of blood of rats with fructose model of metabolic syndrome (MS). White male rats (n=54, with initial body weight of 190-210 g) were randomly divided into five groups: control and 4 experimental groups. Body weight, circumference of the abdomen, blood serum level of glucose, total cholesterol, triglycerides and high density (HDL) lipoproteins were monitored in all animals in dynamics. Also non-specific proteases and their inhibitors were evaluated in rat blood by enzymatic methods, lipid peroxidation profile (malondialdehyde and caeruloplasmin blood serum level, superoxide dismutase blood activity) - by spectrophotometric methods. Modeling of the MS (10% fructose in drinking water) within 8 weeks resulted in statistically significant increase in body mass, abdominal adipose tissue, the activity of elastase-like (ELA) and trypsin-like (TLA) proteinases (20 and 18% respectively), the level of secondary products of LPO (50%), and decrease in activity of superoxide dismutase (15%) compared with the parameters of intact animals. The use of polyphenol-rich materials obtained from grapes with a total content of 1; 1,1 and 4 mg of phenolic compounds (in 0.05 ml) at MS for 4 weeks contributed regression the key clinical signs of MS: significant decrease of glucose and triglyceride levels against the background of increasing the HDL cholesterol were observed. The polyphenol-rich materials obtained from grapes influenced positively on the proteolytic profile (decreased TLA and ELA by 20% at the maximum dosage), contributed to the increase of the level of acid-stable protease inhibitors (by 21%), and reduced the amount of secondary products of LPO (by 34%). The dose-dependent effect of the amount of polyphenol components contained in food concentrates has been revealed.