The influence of diet therapy and regular physical trainings on monocyte chemoattractant protein-1 (MCP-1) secretion by monocytes among obese patients with coronary heart disease
AbstractChronic systemic inflammation is one of the leading pathogenetic pathways for the development of atherosclerosis in obese patients. In this regard, it seems promising to evaluate the effect of the diet and physical exertion on the proinflammatory activity of monocytes.
The purpose of this research was to evaluate the effect of the diet and regular physical trainings on the secretion of monocyte chemotactic factor 1 (MCP-1) by monocytes in obese patients with coronary artery disease.
Material and methods. 27 obese participants (body mass index >30 kg/m2) with a confirmed diagnosis of coronary heart disease were recruited. All participants were prescribed with 12 weeks of a specialized diet with a restriction of simple carbohydrates and salt, a 500-kcal daily energy deficit, and with inclusion of cruciferous (200 g per day), seasonal dark berries (70 g per day) and green tea (200 ml per day). The regular assisted physical trainings were also administered. The body composition, blood biochemical parameters and MCP-1 secretion rates in the primary culture of monocytes isolated from blood samples via the immunomagnetic separation method were assessed before and after the intervention.
Results. As a result, after the 12-weeks intervention the reliable body weight loss (-4.0%), waist circumference (-4.2%), visceral fat (-5.4%), total cholesterol (-9.8%), LDL-cholesterol (-16.6%) and triglycerides (-26.0%), an improvement in the results of the 6-minute walk test (+10.33%) was achieved, as well as an LPS-stimulated monocytes secretion of MCP-1 decreased by 2.8 times (p=0.005).
Conclusion. Overall, the results suggest that diet and regular physical activity in patients with obesity and coronary heart disease may decrease the functional “proinflammatory” activity of monocytes.
Keywords:diet; physical trainings; monocyte chemotactic factor 1; obesity; coronary heart disease
Funding. The study was supported by a grant from the Russian Science Foundation № 22-25-00414, https://rscf.ru/project/22-25-00414/.
Conflict of interest. The authors declare no conflict of interest.
Contribution. Concept and design of the study – Starodubova A.V., Varaeva Yu.R., Kirichenko T.V.; data collection and processing – Shaposhnikova N.N., Varaeva Yu.R., Markina Yu.V., Tolstik T.V.; statistical analysis – Shaposhnikova N.N., Varaeva Yu.R., Kirichenko T.V.; manuscript preparation – Starodubova A.V., Shaposhnikova N.N., Varaeva Yu.R., Kirichenko T.V.; editing – Starodubova A.V., Nikityuk D.B.; approval of the final version of the article, responsibility for the integrity of all parts of the article – all authors.
For citation: Starodubova A.V., Shaposhnikova N.N., Varaeva Yu.R., Kirichenko T.V., Markina Yu.V., Tolstik T.V., Nikityuk D.B. The influence of diet therapy and regular physical trainings on monocyte chemoattractant protein-1 (MCP-1) secretion by monocytes among obese patients with coronary heart disease. Voprosy pitaniia [Problems of Nutrition]. 2024; 93 (2): 63–72. DOI: https://doi.org/10.33029/0042-8833-2024-93-2-63-72 (in Russian)
References
1. URL: http://www.who.int/mediacentre/factsheets/fs317/en/ (date of access September, 2023).
2. Haslam D.W., James W.P. Obesity. Lancet. 2005; 366 (9492): 1197–209. DOI: https://doi.org/10.1016/S0140-6736(05)67483-1
3. Kotova Yu.A., Zuikova A.A. Study of markers of endothelial damage, oxidative and cellular stress in patients with IHD and concomitant obesity. Journal of New Medical Technologies. 2021; (2): 25–8. DOI: https://doi.org/10.24412/1609-2163-2021-2-25-28 (in Russian)
4. Ni Mhurchu C., Rodgers A., Pan W.H., Gu D.F., Woodward M.; Asia Pacific Cohort Studies Collaboration. Body mass index and cardiovascular disease in the Asia-Pacific Region: an overview of 33 cohorts involving 310 000 participants. Int J Epidemiol. 2004; 33 (4): 751–8. DOI: https://doi.org/10.1093/ije/dyh163
5. Manson J.E., Colditz G.A., Stampfer M.J., Willett W.C., Rosner B., Monson R.R., et al. A prospective study of obesity and risk of coronary heart disease in women. N Engl J Med. 1990; 322 (13): 882–9. DOI: https://doi.org/10.1056/NEJM199003293221303
6. Manson J.E., Willett W.C., Stampfer M.J., Colditz G.A., Hunter D.J., Hankinson S.E., et al. Body weight and mortality among women. N Engl J Med. 1995; 333 (11): 677–85. DOI: https://doi.org/10.1056/NEJM199509143331101
7. Gutiérrez-Cuevas J., Sandoval-Rodriguez A., Meza-Rios A., Monroy-Ramírez H.C., Galicia-Moreno M., García-Bañuelos J., et al. Molecular mechanisms of obesity-linked cardiac dysfunction: an up-date on current knowledge. Cells. 2021; 10 (3): 629. DOI: https://doi.org/10.3390/cells10030629
8. Liang W., Qi Ya., Yi H., Mao C., Meng Q., Wang H., et al. The roles of adipose tissue macrophages in human disease. Front Immunol. 2022; 13: 908749. DOI: https://doi.org/10.3389/fimmu.2022.908749
9. Kolb H. Obese visceral fat tissue inflammation: from protective to detrimental? BMC Med. 2022; 20 (1): 494. DOI: https://doi.org/10.1186/s12916-022-02672-y
10. Lee Y., Lee S.-H., Jung E.S., Kim J.-S., Shim C.Y., Ko Y.-G., et al. Visceral adiposity and the severity of coronary artery disease in middle-aged subjects with normal waist circumference and its relation with lipocalin-2 and MCP-1. Atherosclerosis. 2010; 213 (2): 592–7. DOI: https://doi.org/10.1016/j.atherosclerosis.2010.09.012
11. Huang Q., Fei X., Li S., Xu C., Tu C., Jiang L., Wo M. Predicting significance of COX-2 expression of peripheral blood monocyte in patients with coronary artery disease. Ann Transl Med. 2019; 7 (18): 483. DOI: https://doi.org/10.21037/atm.2019.08.75
12. Eraković M., Duka M., Becik M., Tomic S., Ismaili B., Vučević D., et al. Anti‐inflammatory and immunomodulatory effects of Biodentine on human periapical lesion cells in culture. Int Endod J. 2020; 53 (10): 1398–412. DOI: https://doi.org/10.1111/iej.13351
13. Basurto L., Gregory M.A., Hernandez S.B., Sanchez-Huerta L., Martinez A.D., Manuel-Apolinar L., et al. Monocyte chemoattractant protein-1 (MCP-1) and fibroblast growth factor-21 (FGF-21) as biomarkers of subclinical atherosclerosis in women. Exp Gerontol. 2019; 124: 110624. DOI: https://doi.org/10.1016/j.exger.2019.05.013
14. Rotstein A.H., Gibson R.N., King P.M. Direct B‐mode NASCET‐style stenosis measurement and Doppler ultrasound as parameters for assessment of internal carotid artery stenosis. Aust Radiol. 2002; 46 (1): 52–6. DOI: https://doi.org/10.1046/j.1440-1673.2001.00994.x
15. Kurl S., Ravani A., Frigerio B., Sansaro D., Bonomi A., Tedesco C.C., et al. IMPROVE study group. Carotid plaque-thickness and common carotid IMT show additive value in cardiovascular risk prediction and reclassification. Atherosclerosis. 2017; 263: 412–9. DOI: https://doi.org/10.1016/j.atherosclerosis.2017.05.023
16. Ezhov M.V., Kukharchuk V.V., Sergienko I.V., Alieva A.S., Antsiferov M.B., Ansheles A.A. et al. Disorders of lipid metabolism. Clinical Guidelines 2023. Rossiyskiy kardiologicheskiy zhurnal [Russian Journal of Cardiology]. 2023; 28 (5): 250–97. DOI: https://doi.org/10.15829/1560-4071-2023-5471 (in Russian)
17. Kolotov K.A., Rasputin P.G. Monocyte chemotactic protein-1 in physiology and medicine. Permskiy meditsinskiy zhurnal [Perm’ Medical Journal]. 2018; (3): 99–105. DOI: https://doi.org/10.178l6/pmj35399-105 (in Russian)
18. Deshmane S.L., Kremlev S., Amini S., Sawaya B.E. Monocyte chemoattractant protein-1 (MCP-1): an overview. J Interferon Cytokine Res. 2009; 29 (6): 313–26. DOI: https://doi.org/10.1089/jir.2008.0027
19. Santamarina A.B., Pisani L.P., Baker E.J., Marat A.D., Valenzuela C.A., Milesc E.A., et al. Anti-inflammatory effects of oleic acid and the anthocyanin keracyanin alone and in combination: effects on monocyte and macrophage responses and the NF-κB pathway. Food Funct. 2021; 12 (17): 7909–22. DOI: https://doi.org/10.1039/d1fo01304a
20. Arango-Varela S.S., Luzardo-Ocampo I., Maldonado-Celis M.E., Campos-Vega R. Andean berry (Vaccinium meridionale Swartz) juice in combination with Aspirin modulated anti-inflammatory markers on LPS-stimulated RAW 264.7 macrophages. Food Res Int. 2020; 137: 109541. DOI: https://doi.org/10.1016/j.foodres.2020.109541
21. Lee S.G., Brownmiller C.R., Lee S.-O., Kang H.W. Anti-inflammatory and antioxidant effects of anthocyanins of Trifolium pratense (red clover) in lipopolysaccharide-stimulated RAW-267.4 macrophages. Nutrients. 2020; 12 (4): 1089. DOI: https://doi.org/10.3390/nu12041089
22. Melnikov N., Kamari Y., Kandel-Kfir M., Barshack I., Ben-Amotz A., Harats D., et al. β-Carotene from the alga Dunaliella bardawil decreases gene expression of adipose tissue macrophage recruitment markers and plasma lipid concentrations in mice fed a high-fat diet. Mar Drugs. 2022; 20 (7): 433. DOI: https://doi.org/10.3390/md20070433
23. Zhao R., Xiang B., Dolinsky V.W., Xia M., Shen G.X. Saskatoon berry powder reduces hepatic steatosis and insulin resistance in high fat-high sucrose diet-induced obese mice. J Nutr Biochem. 2021; 95: 108778. DOI: https://doi.org/10.1016/j.jnutbio.2021.108778
24. Yu S.Y., Kim M.-B., Park Y.-K., Bae M., Kang Y., Hu S., et al. Anthocyanin-rich aronia berry extract mitigates high-fat and high-sucrose diet-induced adipose tissue inflammation by inhibiting nuclear factor-κB activation. J Med Food. 2021; 24: 586–94. DOI: https://doi.org/10.1089/jmf.2020.0127
25. Varaeva Y.R., Kirichenko T.V., Shaposhnikova N.N., Nikityuk D.B., Starodubova A.V. The role of diet in regulation of macrophages functioning. Biomedicines. 2022; 10 (9): 2087. DOI: https://doi.org/10.3390/biomedicines10092087
26. Duggal N.A., Niemiro G., Harridge S.D.R., Simpson R.J., Lord J.M. Can physical activity ameliorate immunosenescence and thereby reduce age-related multi-morbidity? Nat Rev Immunol. 2019; 19: 563–72. DOI: https://doi.org/10.1038/s41577-019-0177-9
27. Lagzdina R., Rumaka M., Gersone G., Tretjakovs P. Circulating levels of IL-8 and MCP-1 in healthy adults: changes after an acute aerobic exercise and association with body composition and energy metabolism. Int J Mol Sci. 2023; 24 (19): 14725. DOI: https://doi.org/10.3390/ijms241914725
28. Monteiro P. A., Campos E.Z., de Oliveir F.P., Peres F.P., RosaNeto J.C., Pimentel G.D., et al. Modulation of inflammatory response arising from high-intensity intermittent and concurrent strength training in physically active males. Cytokine. 2017; 91: 104–9. DOI: https://doi.org/10.1016/j.cyto.2016.12.007
29. Della Guardia L., Codella R. Exercise tolls the bell for key mediators of low-grade inflammation in dysmetabolic conditions. Cytokine Growth Factor Rev. 2021; 62: 83–93. DOI: https://doi.org/10.1016/j.cytogfr.2021.09.003
30. Evers-van Gogh I.J.A., Oteng A.B., Alex S., Hamers N., Catoire M., Stienstra R., et al. Muscle-specific inflammation induced by MCP-1 overexpression does not affect whole-body insulin sensitivity in mice. Diabetologia. 2016; 59: 624–33. DOI: https://doi.org/10.1007/s00125-015-3822-2
31. Middelbeek R.J.W., Motiani P., Brandt N., Nigro P., Zheng J., Virtanenet K.A., et al. Exercise intensity regulates cytokine and klotho responses in men. Nutr Diabetes. 2021; 11: 5. DOI: https://doi.org/10.1038/s41387-020-00144-x
32. Ihalainen J.K., Schumann M., Eklund D., Hämäläinen M., Moilanen E., Paulsen G., et al. Combined aerobic and resistance training decreases inflammation markers in healthy men. Scand J Med Sci Sports. 2018; 28 (1): 40–7. DOI: https://doi.org/10.1111/sms.12906
33. Boytsov S.A., Pogosova N.V., Ansheles A.A., Badtieva V.A., Balakhonova T.V., Barbarash O.L., et al. Cardiovascular prevention 2022. Russian national guidelines. Rossiyskiy kardiologicheskiy zhurnal [Russian Journal of Cardiology]. 2023; 28 (5): 119–249. DOI: https://doi.org/10.15829/1560-4071-2023-5452 (in Russian)