The study of the association of polymorphism rs659366 gene UCP2 c obesity and type 2 diabetes among residents of the Moscow Region
Abstract1112 people from Moscow region have been surveyed. Genotyping of rs659366 polymorphism UCP2 gene was performed using allele-specific amplification, result detection in real time and using TaqMan-probes complementary DNA polymorphic sites. The study of rs659366 polymorphism of the UCP2 gene has showed that 36,9% of patients had genotype AA, 46,7% – genotype AG, and 16,5% – genotype GG. The frequency of allele A was 60,2%, allele G – 39,8%. BMI, value of fat mass, visceral fat area, serum glucose and triglyceride levels were significantly higher in carriers of A allele in the homozygous and heterozygous state than in carriers of G allele in the homozygous state. Frequency of A allele compared with G allele in obese patients (BMI greater than 30 kg/m 2) was: OR – 1,52; CI (1,24–1,86), p=0,001, and in diabetes mellitus type 2 – OR – 1,22; CI (0,910–1,622), p=0,19.
Keywords:obesity, diabetes mellitus type 2 gene polymorphism rs659366 UCP2