Evaluation of the cytolytic effect of tonic with ginseng extract

• Т.V. Kotova
• А.N. Solopova
• А.S. Sukhikh
• А.S. Razumov
• А.S. Berezina
• V.M. Poznyakovsky

Abstract

Experimental evaluation of the cytolytic effect of prolonged use of tonic drink with ginseng extract by determining the activity of enzymes, markers of cytolysis (alanine aminotransferase, aspartate aminotransferase, creatine kinase and lactate dehydrogenase (LDH)) has been carried out. The study was performed on male rats Wistar (n=15, body weight of 461±23 g), to which tonic drink (containing 46 mg/L panaxosides) was administered in the dose 15 mg/kg body weight using a pharyngeal tube. The introduction was carried out three times daily for 21 days. Then the activity of enzymes - markers of cytolysis was determined in bloodplasma. Three-factor mathematical models predicting changes in LDH activity under prolonged intake of tonic drink with ginseng extract were constructed by regression analysis. The models were characterized by close ties of effective and factorial signs under reliability level of 95%, were adequate by the Fisher test, and the coefficients were significant by Student's test. It was found that a daily intake three-time intake of tonic drink with ginseng extract for 21 days didn't lead to significant changes in the activity of enzymes, markers of cytolysis. This coincided with the forecast estimate of cytolysis using three -factor mathematical models. However, the proposed mathematical models allowed to reveal that changing in the dosage and concentration of the beverage may increase LDH activity corresponding to cytolysis.

Keywords:tonic beverage, ginseng extract, safety, cytolysis markers, three-factor model, regression equation

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